![]() ![]() Mind Screw: Some elements of the setting, especially when the player has their eyes closed or opened (with the dream sequences).Jump Scare: The Husks and the Witch do that throughout different points of the game.Ignorance Is Bliss: From what's implied by the storyline, at least in the case of the endings, especially the "Best Ending".Hell Is That Noise: That odd muffled screech/yell the Husks make.Go Mad from the Revelation: The random glitching when you come across a clue and the lab exploding can be interpreted as this."E.Y.E.S" stands for "Entity's Yielded Essence Storage".V.I.E.W stands for "Vitality and Identity Extraction for WWV".Failure Is the Only Option: When your eyes are stolen, there's a hint that says give up and the other one says, "Don't run away from me.".Evil, Inc.: V.I.E.W seems to be playing this role and, from what's implied (especially by the protest signs and clues left by the Witch), the participants (or many of them) in the Cognitive Transfer program were not entirely willing.Easter Egg: Inputting 666 as the three-digit numerical code on one of the computers will make the computer screen display the sarcastic message, "You're hilarious.". ![]() Dying as Yourself: The Recollection Ending can be interpreted as this. ![]()
0 Comments
![]() They also have article on changing the client’s default folder location for IT admins. You can checkout official article on changing Box Sync folder location. Though the above guide is made on Windows 10, this procedure also applies for Mac and other Windows versions. You can’t change the name of this Sync folder. Hit Done then.Ī folder named “Box Sync” will be created in the changed location. After you have changed to your custom location, review the address in that screen.Select your desired directory and hit OK. Click on Change button under Box Sync section.Click Customize Folder Settings instead of Start Syncing. Once you sign in to Box client, it will show an welcome screen.Guide to Change Box Client’s Default Sync Folder in PC/Mac You can’t change default folder if your administrator already changed the location.Unfortunately once you start syncing, you can’t have the option to change default sync folder.Box Sync is a productivity tool that enables you to mirror data stored on Box to your desktop. You can then navigate and modify content stored on the Box website through your computer’s native file browsing interface, without using a web browser. Once installed, you can save the Box Sync folder somewhere easily accessible on your computer (I would recommend on your desktop or dock if you’re using a Mac). The programs Box Sync (64 bit), Microsoft System CLR Types for SQL Server 2012.macOS Box Sync system requirements for macOSĬontent that syncs down to your computer is available for offline access, and if you make changes to the synced files locally, these changes automatically sync back up to your Box account. To install the Box Sync app on your computer, visit Box Sync for Windows or Box Sync for Mac and follow the instructions for downloading the appropriate app. Download the Box Sync Installer DMG file. Download the latest version of Box Sync for Mac for free. The Secure Download Manager (SDM) is a program for downloading files. Read 24 user reviews and compare with similar apps on MacUpdate. We stand with Ukraine to help keep people safe. Double-click on Box Sync in your Applications folder.Drag the Box Sync app icon into your Applications folder.Open the DMG file when the download is complete.I want my research files there as well, of course. Your synced files are stored in a new folder called Box Sync. After installing Rosetta, click Continue. After you launch Box Drive, Box prompts you to install Rosetta, which is required for Drive to run. Windows Box Sync system requirements for Windows You can access this folder from the Favorites section in the left pane of your Finder window. Download and install Box Drive M1 public beta. Box Sync Serial Key Win/Mac 2022 Box Sync Crack For Windows is an efficient and reliable software solution developed to offer you the means of uploading your most frequently used documents on the cloud (box.com), enabling you to have access to them whenever you need, regardless of your location.MSI file when the download is complete and follow the installation instructions. Box Sync starts automatically when the installation completes. Straightforward and intuitive usage The application features a brief and uneventful installation. ![]() You can access this folder from the Favorites section in the left pane of Windows Explorer. ![]() Alternately, right-click the Box Sync icon in the system tray (bottom right corner of your screen - near the clock) and click Open Sync Folder. ![]() ![]() 2.43.325 APK MOD Menu | Attack multiplier | Very high critical attack | No cooldown | No Mana cost | God mode | No Ads 19.1.1 MOD Menu APK | Damage Multiplier | Defense Multiplier | VIP MOD BLEACH Mobile 3D (TW) / 死神BLEACH-正版授權手遊 100.2.5 MOD Menu APK | Damage Multiplier | Defense Multiplier | PMT VIP MOD BLEACH Mobile 3D (TW) / 死神BLEACH-正版授權手遊 Ver. 39.5.0 MOD Menu APK | Damage Multiplier | Defense Multiplier | PMT VIP MOD BLEACH Mobile 3D (Global) Ver. Open Request Bleach Soul Bankai 1.6.78 MOD 3.0.0 MOD MENU APK | 1 hit kill | God mode | No mana cost | Unlock auto mode Not Working Crossing boundary (Early Access) Ver. Not Working Alien war (Early Access) Ver. 4.0.0 MOD MENU APK | 1 hit kill | God mode | No mana cost | Unlock auto mode 2.43.325 APK MOD Menu | Attack multiplier | Very high critical attack | No cooldown | No Mana cost | God mode ![]() VIP MOD BLEACH Mobile 3D (JP) / BLEACH Soul Rising 50.0.1 MOD Menu APK | Damage Multiplier | Defense Multiplier | PMT VIP MOD BLEACH Mobile 3D (JP) / BLEACH Soul Rising Ver. 1.5 MOD Menu | Damage Multiplier | Defense Multiplier 1.2.3 MOD APK | Menu | Auto Play | God | Instant Die Enemy | Mana | DMG 2.0.20 + 2.0.23 MOD Menu APK | Damage Multiplier | Defense Multiplier | 14.5.11 MOD Menu | God Mode | Weak Enemies | Unlimited Soulbombs | Open Request Bleach: Brave Souls Anime Game List of useful tutorials about how to use this website and its content 12.0.0 MOD Menu APK | God Mode | Weak Enemies | No Skill CD + Costs | Movespeed x4 - Android & iOS MODs, Mobile Games & Apps You are rooted and want to know how to patch your device? Please click here for more information. Specific game account login possible (for example: E-Mail, HIVE, Kakao)? Yes. Once you fill that requirements the process is the same as with signed APKs with the difference that you can overwrite the original game/app with the MOD APK without removing it first.įor the case the unsigned APK does fail to install: Your device patch is not done correctly!įacebook login possible? Yes. This can be done with the help of tools such as Luckypatcher or Xposed. These are not always provided by the mod publisher as they do only work under certain circumstances.Ģ.) Your device must be patched to ignore app signatures. Unsigned APKs do only work on rooted and patched devices/environments. PvP Trick: Start the fight with all cheats OFF, turn ON when battle starts & turn off when battle ends. You can be sure to download quality on .ģ. Stay away from harmful malicious mods that fill your device with UNWANTED ADS! I always provide quality service with no such malicious tricks to earn money. My mod is pretty safe against ban but to run nearly 100% safe use second account and push your real account in co-op or epic raid only. Movespeed don't works in story mode when you have autoplay enabled. Enter battle and earn experience to level up characters and improve their base stats, Ascend them to increase their max level, train them on the Soul Tree to further improve specific stats, or link characters together to provide additional support. Pit your best warriors against other players' teams and vie for the top spot.īrave Souls features a highly flexible character improvement system that allows you to create exactly the warriors and the team you want. Develop your characters as you complete quests that recreate pivotal scenes from the story.Ĭompete in weekly leagues. The possibilities are almost limitless!įollow the Bleach story from the moment Rukia and Ichigo first meet. You can even make combinations of characters you would never see in the original story. Special moves are fully voiced by the original Japanese anime voice actors.Ĭombine your favorite characters into teams of three. Unleash each Bleach character's unique special moves to carve your way to victory. Train them and make your own team with the best players.3D graphics and simple controls make for free-flowing and fast-paced hack-and-slash action. Compete in battles and earn your experience level. Every single character has its own super power and abilities. This game allows you to upgrade your character. It has stages where you fight with your enemies. Follow the bleach story and complete quests to develop your own characters with special abilities. This is the main feature which gives a comprehensive result while playing it. Graphics are pretty nice and high in quality. ![]() This is an animation game with best visual effects which are really good and accurate that makes this game more enjoyable and entertaining. Win your tasks and stages to unlock different characters and useful items. ![]() Challenge other players and fight with the other opponents. You can play this game online with friends and other players around the world. You can never get bored from it because it has a great feature which is multiplayer. ![]() ![]() ![]() 1920x1080px Tank In War Zone Digital, panzer iv HD wallpaper.Tiger Tank, Ww2 Tanks, WW2, German WW2 Tank HD wallpaper 1920x1272px World war 2, video game, soldiers & tanks HD wallpaper.1999x1512px Sherman M-51 Tank, military, isherman, sherman, art, m51, m-51, war, painting, drawing, tank HD wallpaper.1920x1080px Escape From Tarkov City War Zone Video Game Thumbnail HD wallpaper.1280x1024px the last war, abstract, tanks, cg, 3d, statue of liberty, red, war HD wallpaper.Best gaming, Gaming, Game iphone HD phone wallpaper 794x1191px Extreme Gamer Poster Gamer zone War Zone Playstation Art.1920x1200px call of duty world at war, soldier, cod, duty, war, call of duty, gun, tank, world at war, cod5weapon HD wallpaper.1920x1080px The Germans, military, tanks, other,, war HD wallpaper.1920x1080px Battlefield War Zone Fantasy Art, ancient battlefield HD wallpaper.1920x1200px tank war, soldier, war, adventure, action, 3d, abstract, video game, battle, city war, tank, fire HD wallpaper.2000x1318px Desert Tanks, world war 2, eriwn rommel, tanks, quote, desert, nazi, desert fox HD wallpaper.2560x1440px Battlefield 4, cool war zone background HD wallpaper. ![]() 1920x1200px game war, war zone, soldiers, war HD wallpaper.2480x1841px world war 2 tank art, ww2 anime HD wallpaper.1920x1080px war zone backgrounds 9, cool war zone background HD wallpaper.2880x1968px Georgian, tank, war HD wallpaper.1600x809px 12 Characters From The Mahabharata Who Survived The Kurukshetra War, karna mahabharat HD wallpaper.3604x2434px War thunder, video game, military, tanks, aircrafts HD wallpaper.1280x800px tanks during the war, military, aircraft HD wallpaper.1920x1200px Panzer tank, german tank, panzer, german, world war, ww2 HD wallpaper.IPad 3, iPad 4, iPad Air, iPad iPad, iPad Mini 2, iPad Mini 3, iPad Mini 4, 9.7" iPad Pro: 2048x1536, 1536x2048Ĭool war zone background video game war zone Games War Thunder soldiers soldier abstract Tank Tanks Battle Men War MoW People ![]() IPhone 12 Pro Max, iPhone 13 Pro Max, iPhone 14 Plus: 1284x2778 IPhone Xs Max, iPhone 11 Pro Max: 1242x2688 IPhone X, iPhone Xs, iPhone 11 Pro: 1125x2436 IPhone 6 plus, iPhone 6s plus, iPhone 7 plus, iPhone 8 plus: 1242x2208 IPhone 6, iPhone 6s, iPhone 7, iPhone 8: 750x1334 IPhone 5, iPhone 5s, iPhone 5c, iPhone SE: 640x1136 IPhone: iPhone 2G, iPhone 3G, iPhone 3GS: 320x480 Pixel 3xl marvel’s avengers wallpapers is a wallpaper app that was recently released in the app store.MacBook Pro 13.3" Retina, MacBook Air 13" Retina, MacBook Air 13.3"(2020, M1): 2560x1600 Dual monitor: Here are some common misconceptions about pixel 3xl marvel’s avengers wallpapers and why they may not be true:Īpple was already advertising pixel 3xl before it was even released The purpose of these facts about this new app is to provide people with information about this particular app. The purpose of this wallpaper app is to use artificial intelligence (AI) in order to create unique and beautiful wallscapes that would normally take days or weeks to make by hand. This app is a copy of this 1.0 version of pixel avengers: -This is an ad for the google pixel The app doesn’t need access to your camera, it just uses AI to generate beautiful wallpapers. This app was made by a company that produces selfie apps and photo filters, but nothing to do with google or their products. ![]() “Pixel 3xl marvel’s avengers wallpapers is a tool for the google pixel 3 lineup. Pixel 3xl marvel’s avengers wallpapers is a brand new app that was recently released in the app store.Ģ. The newest version of this app can be downloaded for free from the app store. Even though the newest version contains all of the newer features, it will not contain any ads or in-app purchases. The developers would much rather make money from selling their other apps and wallpapers, but they have decided to make this app ad free while they develop and test a few features to improve it in future updates.ģ. This app will be promoted by Apple and others as a fun wallpaper app. The free ad-supported version can be downloaded for free and will not contain any ads or in-app purchases.Ĥ. #Pixel 3xl battlefield v wallpapers for free# ![]() ![]() If you find someone, share your verification code and you’re done. That being said, some messages might not receive a response at all.Īn unconventional way of getting WeChat approval is reaching out to generous folks on online forums like Reddit, asking them if they’re willing to verify your WeChat account. Users report that contacting the support team in Chinese is helpful, but messages sent in English take more time to receive a response. However, in some cases, users have reported that contacting the WeChat support team helps. Have a WeChat user scan the QR code to complete registration. Unfortunately, unless you complete this process, there’s a very slim chance that you’ll be able to access WeChat. Once you do find someone, you’ll need to provide them with your QR code so they can verify you. Finding a China Mainland user might be hard for an international user, but it helps. Nobody knows why WeChat declines some accounts even after verification, but to maximize your chances of approval, see if you can find a friend based in Mainland China to verify your account. Note that WeChat might decline activating your account even if all conditions are met.
![]() Comment online via the following link: Comment nowįrank Pritzlaff, P.E.WisDOT welcomes comments and suggestions related to this study. The Interstate 39/90 Reconstruction Project involves the expansion of the interstate highway to interchange US 12/18 near Madison, from the Illinois State. For more information, please view theĬorridor needs assessment appendices Contact information Further evaluation between Wisconsin Dells and Tomah may be part of a possible future corridor study. The purpose of the study was to provide information to make informed decisions for the I-39/90-/94 corridor regarding potential future safety, pavement, bridge, or other improvement projects. Future year analysis included forecasts for conditions through the year 2050. Evaluations included analysis of traffic, safety, pavement, and structures within the corridor. The study evaluated the existing and future conditions along I-90 corridor from US 12/18 in Madison to the I-90 I-94 split in Tomah (approximately 100 miles), including a portion of I-39 from I-90/94 to WIS 16. WisDOT recently completed I-90 Madison to Tomah Needs Study (January 2022).Currently gathering data to identify corridor alternatives and potential impacts on human-made and natural resources..Public involvement activities are expected to begin in late 2022.Environmental study with no construction activities planned at this time.To view interchange alternatives that were presented at the second Public Involvement Meeting (PIM) CLICK HERE. Status/phase I-39/90/94 Corridor Study – Public involvement page. Project overview The purpose of the project is to address the deteriorating pavement and extend the service life of the concrete driving lanes and asphalt shoulders. Finalize environmental impact analysisįor more information about public involvement opportunities and other activities, visit the Project location I-39/90 (US 12/18 to Lien Road), Madison - Dane County View I-39/90 (US 12/18 to Lien Road) project on a larger map.*Please note, off alignment options are not being considered for this study Schedule The EIS process, which will include ongoing opportunities for public involvement, will lead to the identification of a preferred alternative. WisDOT will identify project needs and evaluate a range of alternatives in an environmental impact statement (EIS).The purpose of the I-39/90/94 Corridor Study is to address existing and future traffic demands, safety issues, aging and outdated infrastructure.The study corridor is about 67 miles long and travels through Dane, Columbia, Sauk and Juneau counties. The study will also evaluate I-39 from where it splits from I-90/94 to Levee Road near Portage. The Wisconsin Department of Transportation (WisDOT) and the Federal Highway Administration (FHWA) are conducting the I-39/90/94 Corridor Study between US 12/18 in Madison and US 12/WIS 16 in Wisconsin Dells.WisDOT in partnership with the City of Madison invites you to attend one of two meetings to discuss improvement alternatives for Madison-area interchanges developed as part of this study:ĬLICK HERE to view the postcard invite! ![]() ![]() ![]() Overall this felt like a huge improvement over what I was seeing with the previous version, where I’d go do some chores, eat dinner, etc… while it churned on 5 big jpeg files. As you can see from the dialog above of the files I passed in, the files still aren’t exactly tiny but both the 32 & 64-bit versions of Photomatix 4.0 only took 42 seconds to process all 5 shots into an HDR image, and 4.1.1 is even faster!Īfter I was done making changes, I clicked the process button which generated the final tone mapped image in only 22 seconds which saved out to 88.9 MB TIFF file when I chose Save Image (again faster in 4.1.1). As a result of this recommendation from the king of HDR, I decided that I’d give up my obsession with passing in the fat RAW files and get with the times. ![]() I’ve enjoyed Trey Ratcliff’s HDR Workshop DVD’s, and in it he still recommends converting your images to JPEG when processing in Photomatix because it is believed that this is what Photomatix does with your RAW files anyway, and its raw conversion isn’t very good – I agree. Gone are the days of being dumped off with a bunch of complex sliders and no clue as to how to get started - woohoo! Performance Improvements Many will find that their new workflow will be to pick the preset that gets them closest to their artistic intent, and then tweak those values as needed in the Adjustments window. The big improvements in 4.1+ come with the long-overdue visual preset thumbnails, and there are plenty of them! The rest of the UI is similar, but more logical so I find it to be a more simplified way of processing your images. Visual presets are a big bonus as are both 64 & 32-bit support The 3.x Workflow Shortcuts window gets new terminology and features, but the 3.x Generate HDR – Options dialog is simplified and renamed to Preprocessing Options. Getting started in Photomatix is the effectively same, but simple & effective user-interface. New Workflow Shortcuts & the renamed Preprocessing Options dialog are your entry points You can click the image to see my mini-review of this lens or click here to purchase it from B&H. At first I had the natural look and then I couldn’t help but go wild with it as this image needed some oomph. Using the latest build of Photomatix I processed this image on August 17th. Here’s one of those wild HDR images created with my new Canon 8-15mm Fisheye Zoom ( review ) Although that has changed over the last year as there’s exciting stuff happening in the HDR world including the release of Photomatix 4.1 – the industry leading solution in HDR processing! or existing owners of Photomatix, this is a no brainer upgrade, and for those who have used the trial version forever, it’s time to pony up and get those watermarks off your HDR images! Sample Image **** Don’t forget to get your 15% discount when you use the coupon code at the end of this article! ****Ī while back I reviewed Photomatix 3.1 Pro Plus, and I must confess that at the time I didn’t really do much HDR or Tone Mapping work. ![]() ![]() ![]() He has 33 actor, 19 writer, 10 director, and five producer credits. Was Peter Kay in Doctor Who? Yes, on 17th June 2006, he appeared in an episode of Doctor Who titled Love & Monsters. He is also actively involved in theatre productions and stand-up comedy shows. The comedian began his television career in the 1990s. Profile summary Full nameīolton, Lancashire, England, United KingdomĬomedian, voice actor, producer, and Kay Interestingly, he would rather be at home with his children and spouse than out doing shows. Peter Kay is a self-proclaimed homebody and family man. The comedian poses in the Winners' Room at the House Of Fraser British Academy Television Awards 2016 at the Royal Festival Hall in London, England. He has appeared in multiple television and film production and authored books. His talent has been praised by fellow comedians and fans. He is ranked among the funniest people in the United Kingdom and the world. Peter Buka’s birth sign is Cancer and he has a ruling planet of Moon.Peter Kay is a gifted comedian, comedy writer, and actor. People who are born with Moon as the ruling planet are sensitive, nurturing, and usually have fluctuating moods. The Moon represents one's nurturing and emotional instinct. Ruling Planet: Peter Buka has a ruling planet of Moon and has a ruling planet of Moon and by astrological associations Sunday is ruled by moon. They have a strong faith and can be seen as stubborn in their old ways. ![]() People born under this sign are persistent and straightforward. The greatest overall compatibility with Cancer is Capricorn and Taurus.Ĭhinese Zodiac: Peter Buka was born in the Year of the Rabbit. The strengths of this sign are being loyal, emotional, sympathetic, tenacious, persuasive, while weaknesses can be moody, pessimistic, suspicious, manipulative and insecure. People of this zodiac sign like home-based hobbies, relaxing near or in water, helping loved ones, and dislike strangers, revealing of personal life. Once more details are available on who he is dating, we will update this section. Like many famous people and celebrities, Peter Buka keeps his personal life private. We will continue to update information on Peter Buka’s parents. Peter Buka’s mother’s name is unknown at this time and his father’s name is under review. The education details are not available at this time. The 25-year-old was born in the Millennials Generation and the Year of the Ox Education They're flexible and good at improvising. They have imagination and don't like planning things in advance. People born on a Monday tend to be a bit sensitive and emotional. While we don't know Peter Buka birth time, but we do know his mother gave birth to his on a Monday. You can also find out who is Peter Buka dating now and celebrity dating histories at CelebsCouples. Having been raised under the mantra "follow your dreams" and being told they were special, they tend to be confident and tolerant of difference. ![]() Millennials is a generation who grew up with computers, internet and social networks. Peter Buka is part of a Millennial Generation (also known as Generation Y). Oh, and the Soviet Union fell and boy bands ruled the music charts. In 1992 the first text message was sent, DVDs were invented, Sony PlayStation was released and Google was founded. The 1990s is often remembered as a decade of peace, prosperity and the rise of the Internet (World Wide Web). JUMP TO: Peter Buka’s biography, facts, family, personal life, zodiac, videos, net worth, and popularity. ![]() ![]() ![]() This means that when the size of humans increases, its volume also increases dramatically in proportion to the size. Likewise, the volume scales up in proportion to the cube of the area's size. The area is directionally proportional to the square of the size of any object. The fake archaeological discovery cannot happen because of the square-cube law, which states that as the size of any object grows, its volume grows faster than its surface area. This was noticed by other Twitter users commenting that even in their design classes, the picture will not pass and even gave a piece of advice to clean up the picture with stamp.Īs it turns out, the photo was from a competition way back in 2002 which asked contestants to create a fake archaeological discovery using photoshop. The user even attached a badly photoshopped picture. qmB5sYGkns - Stodgy Seagull July 8, 2020 This would not pass in my design classes. While some accepted the theory, but there are still many who are making fun of it. What do you believe? /DhiHcxn0kA - Reality is the only check I get July 7, 2020 Because having to explain how they existed contradicted the evolution of mankind from apes. The Vatican and The Smithsonian destroyed most and threw some into the bottom of the ocean. Debunking the Conspiracy Theory Using Square-Cube LawĪccording to a Twitter post by user Reality is the only check I get the Vatican and the Smithsonian destroyed most and threw some of the giant skeletons they found into the ocean since having to explain how they existed contradicts the evolution of mankind. They even provided evidence in the form of the worst photoshop that will not even pass to any graphic artist. ![]() Is Vatican Hiding the Truth of the Existence of the Giant Humans?Īccording to these conspiracy theories, Vatican - in conjunction with the Smithsonian, no less - is hiding the truth about the existence of the gigantic human skeletons, which confirms their existence and therefore messing up the evolutionary tree of humans. ![]() ![]() ![]() Interactions between DA and NE theoretically depend on the timing of release, spatial diffusions, concentrations, and DA/NE ratios. Furthermore, dysfunction of dopaminergic or noradrenergic systems are thought to be associated with psychiatric disorders and neurodegenerative diseases, such as attention-deficit/hyperactivity disorder (ADHD), schizophrenia, and Parkinson’s disease. In particular, the prefrontal cortex receives both dopaminergic and noradrenergic projections, and these systems are involved in attention and working memory. In addition, the involvement of DA and NE overlap in many brain functions, such as learning and memory, arousal, and stress response. DA is involved in reward, motivation, novelty response, and motor control. It was discovered recently that noradrenergic LC axons co-released DA along with NE. Dopaminergic projections mainly originate from the ventral tegmental area and the substantia nigra pars compacta, whilst noradrenergic projections mainly originate from the locus coeruleus (LC). The catecholaminergic neuromodulators dopamine (DA) and norepinephrine (NE) have very high structural similarity, differing only by a single hydroxy group. Thus, this is a first step toward the multiplex imaging of these neurotransmitters in, for example, freely moving animals, which will provide new opportunities to advance our understanding of the high spatiotemporal dynamics of DA and NE in normal and abnormal brain function. Taking advantage of the high selectivity of R-GenGAR-DA1.2, we monitored DA in presence of NE using dual-color fluorescence live imaging, combined with the green-NE biosensor GRAB NE1m, which has high selectivity for NE over DA (> 350-fold) in HeLa cells and hippocampal neurons grown from primary culture. R-GenGAR-DA1.2 demonstrated a reasonable dynamic range ( ΔF/F 0 = − 43%), DA affinity (EC 50 = 0.92 µM) and high selectivity for DA over NE (66-fold) in HeLa cells. We developed two variants: R-GenGAR-DA1.1, which brightened following DA stimulation, and R-GenGAR-DA1.2, which dimmed. More specifically, a circular permutated red fluorescent protein (cpmApple) was replaced by the third intracellular loop of human DA receptor D1 (DRD1) followed by the screening of mutants within the linkers between DRD1 and cpmApple. Here, we report the development of a red fluorescent genetically encoded GPCR (G protein-coupled receptor)-activation reporter for DA termed ‘R-GenGAR-DA’. Although genetically encoded fluorescent biosensors have been developed to detect DA, their poor selectivity prevents distinguishing DA from NE. Despite their physiological importance, untangling the relationship between DA and NE in the fine control of output function is currently challenging, primarily due to a lack of techniques to allow the observation of spatiotemporal dynamics with sufficiently high selectivity. Dopamine (DA) and norepinephrine (NE) are pivotal neuromodulators that regulate a broad range of brain functions, often in concert. ![]() |